CHROMOSOME NUMBERS, MEIOTIC ABNORMALITIES, AND 2N POLLEN FORMATION IN ACCESSIONS OF THE WILD SPECIES CHRYSOLAENA FLEXUOSA (SIMS) H. ROB. (VERNONIEAE, COMPOSITAE) FROM ITS DISTRIBUTION RANGE IN ARGENTINA

CHROMOSOME NUMBERS, MEIOTIC ABNORMALITIES, AND 2N POLLEN FORMATION IN ACCESSIONS OF THE WILD SPECIES CHRYSOLAENA FLEXUOSA (SIMS) H. ROB. (VERNONIEAE, COMPOSITAE) FROM ITS DISTRIBUTION RANGE IN ARGENTINA
16 septiembre, 2017 Sociedad argentina de botánica

Boletín de la Sociedad Argentina de Botánica

MARÍA LIS ECHEVERRÍA, ELSA LUCILA CAMADRO

 CHROMOSOME NUMBERS, MEIOTIC ABNORMALITIES, AND 2N POLLEN FORMATION IN ACCESSIONS OF THE WILD SPECIES CHRYSOLAENA FLEXUOSA (SIMS) H. ROB. (VERNONIEAE, COMPOSITAE) FROM ITS DISTRIBUTION RANGE IN ARGENTINA”

 Recibido 16/02/2017
Aceptado 28/04/2017

Summary: Chrysolaena flexuosa is a South American species of potential ornamental value (basic chromosome number of x=10). Diploid (n=10) and tetraploid (n=20) cytotypes have been reported for its distribution area although one hexaploid (n=30-32ca.) cytotype has been reported for its most southern distribution. To investigate if ploidy and latitude are positively related in Ch. flexuosa natural populations and if sexual polyploidization could have had a role in the origin of the polyploid cytotypes, we determined chromosome numbers, DNA content, and pollen viability and size and analyzed microsporogenesis in samples of seven Argentinian accessions. Two of the northeastern accessions were diploid and one was tetraploid, whereas the four southeastern accessions were hexaploid. Ploidy levels determined both by chromosome countings and flow cytometry coincided, although monoploid genome size significantly decreased with increasing ploidy. In all accessions, variability was observed for pollen viability and size, as well as for large (presumably 2n) pollen production. This variability was underlined by abnormal meiotic events (lagging chromosomes, parallel spindles, triads at the tetrad stage). The results would indicate that there is, apparently, a positive relation between ploidy and latitude, and suggest a likely role of sexual polyploidization in the origin, establishment and expansion of Ch. flexuosa populations.

Keywords: polyploid cytotypes, DNA content, flow cytometry, meiotic abnormalities, abnormal spindle orientation, 2n pollen

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